BIO203 GDB Fall 2023 – GDB – Solution VU


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BIO203 GDB Fall 2023 solution idea:


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Q: Difference among Southern Northern and Western Blotting;


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Solution:
 

Southern Blot:

Southern blotting is done to detect DNA sequences.

A Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples.

Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.

 

Western Blot:

A western blot is used for the detection of specific proteins in complex samples.


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Proteins are first separated by size using electrophoresis before being transferred to an appropriate blotting matrix (usually polyvinylidene fluoride or nitrocellulose) and subsequent detection with antibodies.

 

Northern Blot: 

Northern blotting is performed to detect RNA sequences. The northern blot is for the detection of specific RNA sequences in complex samples.

Northern blotting first separates samples by size via gel electrophoresis before they are transferred to a blotting matrix and detected with labeled RNA probes.

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