BIO502 Assignment 2 Spring 2023 – Assignments – Solution VU

BIO502 Assignment 2 Spring 2023 – Assignments – Solution VU


BIO 502- Genomics

Assignment # 2 Spring 2023

Question # 1: – What is RNA Splicing?

RNA Splicing is a form of RNA Processing in which newly synthesized RNA is converted into mature RNA in a way that forgets the intricate sequence of introns.

  • The RNA Splicing program consists of removing unencrypted or introns and becoming a member of the coding or exon sequence. RNA Splicing takes place regionally or immediately after transcription within the gene embedded within the nucleus.
  • In eukaryotic cells, RNA Splicing is important because it ensures that the unripe RNA molecule is converted into a mature molecule that can then be translated into Proteins.
  • Post transcription trading is not important for prokaryotic cells.
  • RNA Splicing is a controlled system controlled by the beneficial resources of various Ribo-nucleo Proteins.

Question # 2: – How the Splicing Site Determined?

Spliceosome elements see a unique sequence at the edges of the introns called splice websites. So in reality, these are areas in the RNA messenger line where the molecule can be cut and regenerated during the law of protein synthesis by cells.

  • Exons and splice sites are defined with the help of communication for all officials, within the U1 snRNP and U2AF.
  • SR proteins are bound to ESEs and stimulate the synthesis of these compounds.
  • Introns are removed from the primary text by being stored in a stored sequence called splice sites. These sites are cut at the 5th and 3rd edge of the introns.

At the highest level, the RNA emissions begin with Di-nucleotide GU at its 5th end, and end with AG at 3 stops.

Types: –

  • There are two types of fiber splicing
  • Machinery Cuts
  • Fusion integration

Question # 3: – What is Intron Removal?

Introns are removed by RNA Splicing as RNA matures, which means they are now not produced in the final product of the RNA (mRNA) messenger, while exons continue to bind together as a means of building mature mRNA. Introns can be remembered as the intervention sequence and exons as the sequence displayed

  • If the strands are not removed RNA can be converted to an inactive protein. Cutting occurs in the nucleus before RNA moves to the cytoplasm.
  • The entrance site within introns has a UAUAAC compliance sequence. In most cases U can be changed with the help of C and A can be changed using G. However, the last residue of A is completely preserved (unchanged). The organizational intron 1 requires an external guanosine nucleotide that acts as a nucleophile to attack 5-phosphate five-nucleotide introns. The 3-5exon stop is called the splice supplier. The three OHs in the release of the next three exon sponsors attack the splice acceptor website in a 5-nucleotide three-phase release-release and attach together two contracts together the processing of black pre / rRNA takes the region into the prokaryotic nucleus, and the lack of a nuclear membrane allows for translation while writing is not yet complete. Pre-mRNA splicing to remove intronic sequenes and polyadenylation 3-end pre-mRNA.

Question # 4: – Describe Spliceosome: –

Spliceosomes are described as one of the most complex macromolecular gadgets considered to be three hundred different proteins and five RNAs. The animation tells us that it is this magnificent gadget that works on precursor m RNA cuts unencoded intons and stitches together coding exons.

  • Spliceosome is a large and complex cellular device that is highly visible within the cut-off pieces of a moving nucleus of eukaryotic cells. The Spliceosome is composed of snRNAs and protein complexes. Spliceosomes remove introns from pre-m written RNA, a type of primary text. This process is called splicing. The most efficient eukaryotes have Spliceosomes and metazoans have second Spliceosomes, smaller Spliceosomes.
  • Introns may be longer in healthy eukaryotes, reaching up to multiple structures and sometimes comprising 90% of precursor mRNA. In the experiment came down to leavenary eukaryotes containing a few short strands, usually less than 300 bases at a time. due to the fact that the introns are non-genetic components that are extracted from mRNA before it is directly translated into protein.

Question # 5 Review the Splicing Pathway?

In the process of cutting, some stairs may be separated in sequence or may be retreated. However a well-known method shows collection events performed with the help of a Spliceosome to suppress the repetitive response within a cell.

First of all, the 5 ’splice web page is known using U1 snRNP. Within this molecule, U4 and U6 snRNPs are grouped together in a way that binds the corresponding base between their RNA components, whereas the U5 snRNP is freely associated with protein-protein synthesis. With the entry of tri-snRNP, the complex A is converted into a complex B.

In the next step, the U1 leaves that problem, and the U6 takes its place on the website ‘of five splice online. This requires that the low pairing between U1 snRNA and pre-mRNA is broken, allowing U6 RNA to partially attach. These steps complete the assembly process. The same rearrangement is similar to ensuring that the RNA substrate is well placed to work with it.

Possibly, this method reduces the risk of inconsistent duplication. Connecting the construction of an active site to the successful completion of the first steps in a Spliceosome assembly makes it appear perhaps that a functional website is easily accessible from valid splice websites.

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